Composite

Part:BBa_K2562006:Design

Designed by: Marina Takehana   Group: iGEM18_ASIJ_Tokyo   (2018-10-01)


SERPINA1 secretion using an OsmY tag, His tag, a GFP reporter system, and single terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 591
    Illegal NheI site found at 614
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 209
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1269


Design Notes

For protein purification, nickel lined chromatography tubes were used for this because of the His tag.


Source

SERPINA1, GFP

References